Reverse Transcription:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
SYBR Green Assay:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
Real-time Polymerase Chain Reaction:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
Gene Expression:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
Isolation:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
RNA Library Preparation:Article Title: IL-21 shapes the B cell response in a context-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Glutamine MSKCC Media Preparation Core Facility N/A Lenti-XTM Concentrator Takara Bio 631232 MEM Non-Essential Amino Acids Solution Thermo Fisher 11140050 NP-BSA, Ratio >20 Biosearch Technologies N-5050H-10 NP-BSA, Ratio 5-9 Biosearch Technologies N-5050L-10 NP-CGG Biosearch Technologies N-5055E-5 NP-KLH Biosearch Technologies N-5060-5 NP-PE Biosearch Technologies N-5070-1 Opti-MEM media Thermo Fisher 31985070 pA/G-MNase Cell Signaling Technology 57813 PBS with Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PBS without Ca2+ and Mg2+ MSKCC Media Preparation Core Facility N/A PEI MAX Polysciences 24765 Penicillin:Streptomycin Solution Gemini Bio 400-109-100 Phosphoric acid Sigma-Aldrich 695017 Polybrene Sigma-Aldrich TR-1003-G Potassium bicarbonate Sigma-Aldrich 237205 rmIL-21 R&D Systems 594-ML rmIL-4 R&D Systems 404-ML RPMI-1640 Media MSKCC Media Preparation Core Facility N/A Saccharomyces cerevisiae spike-in DNA Cell Signaling Technology 29987 Saponin Thermo Fisher A18820.14 Sodium pyruvate Thermo Fisher 11360070 Spermidine Cell Signaling Technology 27287 Tamoxifen Sigma-Aldrich T5648 TMB solution Thermo Fisher 00420156 Tween 20 Fisher Scientific BP337-500 Zombie NIR Fixable Viability Dye BioLegend 423106 Zombie Red Fixable Viability Dye BioLegend 423110 Zombie Yellow Fixable Viability Dye BioLegend 423104 Critical commercial assays SuperScript IV reverse transcriptase kit Thermo Fisher 18090010 Quick-RNA Miniprep Kit Zymo Research R1054 PowerUP SYBR Green master mix Applied Biosystems A25742 TaqMan Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 TaqMan Gene Expression Assay Probe (Aicda) Thermo Fisher Mm01184115_m1 TaqMan Gene Expression Assay Probe (Ubc) Thermo Fisher Mm01201237_m1 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB E7490L xGen Broad-Range RNA Library Preparation Kit Integrated DNA Technologies 10009865 xGen UDI Primer Plate 2, 8nt, 96rxn Integrated DNA Technologies 10009816 BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD 554714 Qiagen MinElute Kit QIAGEN 28004 KAPA HTP Library Preparation Kit KAPA Biosystems KK8234 (Continued on next page) Cell Reports 44, 115190, January 28, 2025 19 ..
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